Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

MiR-33a-5p negatively regulates ovariectomy-induced osteoporosis in rats by decreasing WNT10B

Ziqi Cheng , Yapei Lu

Department of Gynecology, The First Hospital of Hangzhou Fuyang, Fuyang First Hospital Affiliated to Binjiang College of Zhejiang Chinese Medical University, Hangzhou, China;

For correspondence:- Ziqi Cheng Email: chengziqi@163.com Tel:+8615888813572

Accepted: 26 May 2023 Published: 30 June 2023

Citation: Cheng Z, Lu Y. MiR-33a-5p negatively regulates ovariectomy-induced osteoporosis in rats by decreasing WNT10B. Trop J Pharm Res 2023; 22(6):1181-1187 doi: 10.4314/tjpr.v22i6.6

© 2023 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the role and potential mechanism of action of microribonucleic acid (miR)-33-5p in osteoporosis after ovariectomy.

Methods: A postmenopausal osteoporosis model was established using an ovariectomized (OVX) rat. Primary bone marrow-derived mesenchymal stem cells (BMMSCs) were obtained from female rats, and were transfected with mimics or inhibitors. Reverse transcription-polymerase chain reaction (RT-PCR) and western blotting were preformed to determine the level of miR-33a-5p and target proteins, while luciferase reporter assay was used to verify whether WNT10B is a target of miR-33a-5p. Micro-computed tomography (micro-CT) analysis was employed to evaluate bone formation.

Results: Bone mineral density (BMD), determined by micro-CT, was significantly decreased in OVX group. MiR-33a-5p was in negative correlation with WNT10B after surgery, and WNT10B was found to be a target of miR-33a-5p. Additionally, inhibition of miR-33a-5p facilitated osteogenic differentiation in vitro, and promoted bone formation in vivo.

Conclusion: Inhibition of miR-33a-5p accelerates bone formation by increasing WNT10B levels. Thus, miR-33a-5p is a potential target for the treatment of osteoporosis.

Keywords: Postmenopausal, Osteoporosis, Ovariectomy, Bone marrow-derived mesenchymal stem cells (BMMSCs), Bone mineral density